Part:BBa_K2943007:Design

First part of the R2 - pyocin cluster

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

we added extra 20 base pairs at the beginning of the cluster that overlaps with the backbone of the plasmid, in order to compose them together in Gibson assembly reaction.

Source

this part is originally found in the genome of the Pseudomonas aeruginosa bacterium.